mKate
mKate (Ex-Max 584 nm/Em-Max 630 nm)
<html>mKate is a monomeric red fluorescent protein with excitation and emission maximums of 588 nm and 635 nm, respectively. mKate was first derived from <i>Entacmaea quadricolor.</i></html>
Excitation & emission spectra
Key facts
- Excitation maximum
- 588 nm
- Emission maximum
- 635 nm
- Optimal laser line
- 561
- Optimal filter
- 630/69
- Extinction coefficient
- 45000
- Quantum yield
- 0.33
mKate has an excitation maximum at 588 nm and an emission maximum at 635 nm, a Stokes shift of 47 nm. Its extinction coefficient is 45000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is mKate?
- <html>mKate is a monomeric red fluorescent protein with excitation and emission maximums of 588 nm and 635 nm, respectively. mKate was first derived from <i>Entacmaea quadricolor.</i></html>
- What laser excites mKate?
- mKate is optimally excited by the 561 laser line. Its excitation maximum is 588 nm.
- What filter should I use to detect mKate?
- A 630/69 filter captures the peak of the mKate emission near 635 nm with minimal spillover.