SureLight RPC
SureLight RPC (Ex-Max 555 nm/Em-Max 635 nm)
SureLight RPC ( R-Phycocyanin) from Columbia Biosciences is a fluorescent protein with an excitation peak at 555 and 620nm nm and an emission peak at 635 nm. SureLight RPC is available in antibody conjugation kits for use in flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 555 nm
- Emission maximum
- 635 nm
- Optimal laser line
- 488-561
- Optimal filter
- 630/69
- Relative brightness
- 4 of 5
- Molecular weight
- 103000
- Commercial conjugates
- 6 from 1 supplier
SureLight RPC has an excitation maximum at 555 nm and an emission maximum at 635 nm, a Stokes shift of 80 nm. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is SureLight RPC?
- SureLight RPC ( R-Phycocyanin) from Columbia Biosciences is a fluorescent protein with an excitation peak at 555 and 620nm nm and an emission peak at 635 nm. SureLight RPC is available in antibody conjugation kits for use in flow cytometry.
- What laser excites SureLight RPC?
- SureLight RPC is optimally excited by the 488-561 laser line. Its excitation maximum is 555 nm.
- What filter should I use to detect SureLight RPC?
- A 630/69 filter captures the peak of the SureLight RPC emission near 635 nm with minimal spillover.
- How bright is SureLight RPC?
- SureLight RPC has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to SureLight RPC?
- FluoroFinder tracks 6 commercially available SureLight RPC conjugates, searchable by target, clone, host and application.