Live/Dead Fix Blue
Live/Dead Fix Blue (Ex-Max 341 nm/Em-Max 440 nm)
The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Blue has an excitation peak at 341 nm and an emission peak at 440 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 341 nm
- Emission maximum
- 440 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
Live/Dead Fix Blue has an excitation maximum at 341 nm and an emission maximum at 440 nm, a Stokes shift of 99 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Live/Dead Fix Blue?
- The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Blue has an excitation peak at 341 nm and an emission peak at 440 nm.
- What laser excites Live/Dead Fix Blue?
- Live/Dead Fix Blue is optimally excited by the 355 laser line. Its excitation maximum is 341 nm.
- What filter should I use to detect Live/Dead Fix Blue?
- A 450/50 filter captures the peak of the Live/Dead Fix Blue emission near 440 nm with minimal spillover.