Loading the Dye Directory…

PromoFluor 350

PromoFluor 350 (Ex-Max 353 nm/Em-Max 431 nm)

PromoFluor 350 is a fluorophore with an excitation peak at 338 nm and an emission peak at 436 nm. It is equivalent to Alexa Fluor™ 350 and AMCA in size, brightness and application. It can be used with the following analysis platforms: Fluorescence Microscopy.

Excitation & emission spectra

400450500550600650700750800

Key facts

Excitation maximum
353 nm
Emission maximum
431 nm
Optimal laser line
355
Optimal filter
450/50
Relative brightness
1 of 5
Quantum yield
0.837

PromoFluor 350 has an excitation maximum at 353 nm and an emission maximum at 431 nm, a Stokes shift of 78 nm. It is optimally excited by the 355 laser line.

Frequently asked questions

What is PromoFluor 350?
PromoFluor 350 is a fluorophore with an excitation peak at 338 nm and an emission peak at 436 nm. It is equivalent to Alexa Fluor™ 350 and AMCA in size, brightness and application. It can be used with the following analysis platforms: Fluorescence Microscopy.
What laser excites PromoFluor 350?
PromoFluor 350 is optimally excited by the 355 laser line. Its excitation maximum is 353 nm.
What filter should I use to detect PromoFluor 350?
A 450/50 filter captures the peak of the PromoFluor 350 emission near 431 nm with minimal spillover.
How bright is PromoFluor 350?
PromoFluor 350 has a relative brightness of 1 out of 5 (quantum yield 0.837).