Dyes similar to MemBrite Fix 405/430 based on emission7-Amino-4-methylcoumarin (AMC) AMCA CellBrite Blue CellBrite Steady 405 CF405S CoraLite 405S CytoTrack Blue DyLight 350 eBFP FVS440UV Ghost Dye UV 450 HiLyte 405 Hoechst (34580) iFluor 405 Live/Dead Fix Blue MaxLight 405 MitoView 405 PromoFluor 350 PromoFluor 405 StarBright UltraViolet 445 StarBright Violet 440 SuperBright 436 SuperNova v428 Vybrant DyeCycle Violet Zombie Violet
About MemBrite Fix 405/430
Biotium’s MemBrite Fix and Membrite Fix-ST dyes are cell surface staining dyes that irreversibly react with cell surface proteins to stain the outer membrane. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and imaging techniques such as STORM. This dye line shares similarities with Biotium’s CellBrite line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.