MemBrite Fix 405/430
MemBrite Fix 405/430 (Ex-Max 411 nm/Em-Max 431 nm)
Biotium's MemBrite Fix and Membrite Fix-ST dyes are amine-reactive cell surface staining dyes that conjugate cell surface proteins to stain the outer membrane of live cells. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and Fluorescence Microscopy techniques such as STORM. MemBrite Fix 405/430 has an excitaion peak at 411 nm and an emission peak at 431 nm and are recommended for use in fluorescence microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 411 nm
- Emission maximum
- 431 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Commercial antibodies
- 2 from 1 supplier
MemBrite Fix 405/430 has an excitation maximum at 411 nm and an emission maximum at 431 nm, a Stokes shift of 20 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is MemBrite Fix 405/430?
- Biotium's MemBrite Fix and Membrite Fix-ST dyes are amine-reactive cell surface staining dyes that conjugate cell surface proteins to stain the outer membrane of live cells. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and Fluorescence Microscopy techniques such as STORM. MemBrite Fix 405/430 has an excitaion peak at 411 nm and an emission peak at 431 nm and are recommended for use in fluorescence microscopy.
- What laser excites MemBrite Fix 405/430?
- MemBrite Fix 405/430 is optimally excited by the 405 laser line. Its excitation maximum is 411 nm.
- What filter should I use to detect MemBrite Fix 405/430?
- A 450/50 filter captures the peak of the MemBrite Fix 405/430 emission near 431 nm with minimal spillover.
- How many antibodies are available conjugated to MemBrite Fix 405/430?
- FluoroFinder tracks 2 commercially available MemBrite Fix 405/430 conjugates, searchable by target, clone, host and application.