CoraLite Plus 405
CoraLite 405S (Ex-Max 404 nm/Em-Max 431 nm)
CoraLite Plus 405 from Proteintech has an excitation peak at 404 nm and an emission peak at 431 nm. It is spectrally similar to DyLight™ 405 and Alexa Fluor™ 405 and can be useful in fluorescence microscopy and flow cytometry. CoraLite® Plus dyes are chemically engineered with SuperHydrophilic dPEG® groups for greater biocompatibility, high water solubility, enhanced brightness and photostability. The family of CoraLite® Plus fluorophores are spectrally similar to Alexa Fluor® dyes.
Excitation & emission spectra
Key facts
- Excitation maximum
- 404 nm
- Emission maximum
- 431 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Relative brightness
- 2 of 5
- Extinction coefficient
- 35000
- Commercial conjugates
- 100 from 1 supplier
CoraLite Plus 405 has an excitation maximum at 404 nm and an emission maximum at 431 nm, a Stokes shift of 27 nm. Its extinction coefficient is 35000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is CoraLite Plus 405?
- CoraLite Plus 405 from Proteintech has an excitation peak at 404 nm and an emission peak at 431 nm. It is spectrally similar to DyLight™ 405 and Alexa Fluor™ 405 and can be useful in fluorescence microscopy and flow cytometry. CoraLite® Plus dyes are chemically engineered with SuperHydrophilic dPEG® groups for greater biocompatibility, high water solubility, enhanced brightness and photostability. The family of CoraLite® Plus fluorophores are spectrally similar to Alexa Fluor® dyes.
- What laser excites CoraLite Plus 405?
- CoraLite Plus 405 is optimally excited by the 405 laser line. Its excitation maximum is 404 nm.
- What filter should I use to detect CoraLite Plus 405?
- A 450/50 filter captures the peak of the CoraLite Plus 405 emission near 431 nm with minimal spillover.
- How bright is CoraLite Plus 405?
- CoraLite Plus 405 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to CoraLite Plus 405?
- FluoroFinder tracks 100 commercially available CoraLite Plus 405 conjugates, searchable by target, clone, host and application.