Live/Dead Fix Red
Live/Dead Fix Red (Ex-Max 593 nm/Em-Max 610 nm)
The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Red has an excitation peak at 593 nm and an emission peak at 610 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 593 nm
- Emission maximum
- 610 nm
- Optimal laser line
- 561
- Optimal filter
- 610/20
Live/Dead Fix Red has an excitation maximum at 593 nm and an emission maximum at 610 nm, a Stokes shift of 17 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is Live/Dead Fix Red?
- The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Red has an excitation peak at 593 nm and an emission peak at 610 nm.
- What laser excites Live/Dead Fix Red?
- Live/Dead Fix Red is optimally excited by the 561 laser line. Its excitation maximum is 593 nm.
- What filter should I use to detect Live/Dead Fix Red?
- A 610/20 filter captures the peak of the Live/Dead Fix Red emission near 610 nm with minimal spillover.