Texas Red
Texas Red (Ex-Max 596 nm/Em-Max 615 nm)
Texas Red (Sulforhodamine 101 acid chloride) is an orange/red emitting dye with an excitation peak at 596 nm and an emission peak at 615 nm. Alternatives to Texas Red that can offer increased photostability and brightness include Alexa Fluor™ 594 (ThermoFisher Scientific), DyLight™ 594 (ThermoFisher Scientific), or CF®594 (BD Biosciences). Texas Red is most commonly used in cellular fluorescence microscopy applications, as the 594 nm laser is rare in flow cytometry. However, it can be sub-optimally excited by the 561 nm yellow-green laser. Texas Red is commonly used as an acceptor in the tandem fluorophore, PE-Texas Red also called ECD.
Excitation & emission spectra
Key facts
- Excitation maximum
- 596 nm
- Emission maximum
- 615 nm
- Optimal laser line
- 594
- Optimal filter
- 610/20
- Relative brightness
- 3 of 5
- Extinction coefficient
- 85000
- Quantum yield
- 0.93
- Molecular weight
- 625
- Commercial antibodies
- 653 from 21 suppliers
Texas Red has an excitation maximum at 596 nm and an emission maximum at 615 nm, a Stokes shift of 19 nm. Its extinction coefficient is 85000 M⁻¹cm⁻¹. It is optimally excited by the 594 laser line.
Frequently asked questions
- What is Texas Red?
- Texas Red (Sulforhodamine 101 acid chloride) is an orange/red emitting dye with an excitation peak at 596 nm and an emission peak at 615 nm. Alternatives to Texas Red that can offer increased photostability and brightness include Alexa Fluor™ 594 (ThermoFisher Scientific), DyLight™ 594 (ThermoFisher Scientific), or CF®594 (BD Biosciences). Texas Red is most commonly used in cellular fluorescence microscopy applications, as the 594 nm laser is rare in flow cytometry. However, it can be sub-optimally excited by the 561 nm yellow-green laser. Texas Red is commonly used as an acceptor in the tandem fluorophore, PE-Texas Red also called ECD.
- What laser excites Texas Red?
- Texas Red is optimally excited by the 594 laser line. Its excitation maximum is 596 nm.
- What filter should I use to detect Texas Red?
- A 610/20 filter captures the peak of the Texas Red emission near 615 nm with minimal spillover.
- How bright is Texas Red?
- Texas Red has a relative brightness of 3 out of 5 (quantum yield 0.93).
- How many antibodies are available conjugated to Texas Red?
- FluoroFinder tracks 653 commercially available Texas Red conjugates, searchable by target, clone, host and application.