405

Laser Line

610/20

Common Filter

407

Excitation Max

602

Emission Max

1,570

Antibodies

1

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About Brilliant Violet 605


Brilliant Violet™ 605 (BV605) is an orange/red-emitting tandem fluorophore that can be excited by the 405 nm violet laser and collected using a 610/20 bandpass filter. BV605 has an excitation peak at 405 nm and an and emission peak at 603 nm. Due to its unique stoke’s shift, not many dyes have identical excitation and emission spectrums, but the nearest alternatives include SuperBright 600, Qdot™ 605 and eFluor™ 605NC (ThermoFisher). BV605 exhibits a medium level of brightness and is most often used in flow cytometry, specifically, it is an alternative to nanocrystals used for intracellular flow. This dye is part of the Brilliant Violet dye line. The Brilliant Violet dye line itself is very popular because other dyes in the line also use the Brilliant Violet 421 core as the donor molecule, while varied acceptor molecules emit light at various points across the visible light spectrum. This allows for multiple dyes to be compatible while excited by the single laser. In cytometry, a user may populate all the available channels off their 405-violet laser with Brilliant Violet dyes.


Popular Brilliant Violet 605 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BioLegend
305430
FC CD86
Human,African Green Monkey,Baboon,Capuchin Monkey,Common Marmoset,Cotton-topped Tamarin,Chimpanzee,Cynomolgus,Rhesus
Mouse
IgG2b κ
IT2.2
100 Tests
$429
View
BioLegend
100237
FC CD3
Mouse
Rat
IgG2b κ
17A2
125 μl
$209
View
BioLegend
123133
FC F4/80
Mouse
Rat
IgG2a κ
BM8
125 μl
$226
View
BioLegend
128035
FC Ly-6C
Mouse
Rat
IgG2c κ
HK1.4
125 μl
$209
View
BioLegend
117334
FC CD11c
Mouse
Armenian Hamster
IgG
N418
50 μg
$286
View
BioLegend
100744
FC CD8a
Mouse
Rat
IgG2a κ
53-6.7
50 μg
$286
View
BioLegend
101257
FC CD11b
Mouse,Human,Chimpanzee,Baboon,Cynomolgus,Rhesus,Rabbit
Rat
IgG2b κ
M1/70
50 μg
$270
View
BioLegend
107639
FC MHC II
Mouse
Rat
IgG2b κ
M5/114.15.2
50 μg
$286
View
BioLegend
506541
ICS IFN-gamma
Human,Chimpanzee,Baboon,Cynomolgus,Rhesus,African Green Monkey,Pigtailed Macaque,Non-Human Primate
Mouse
IgG1 κ
B27
25 Tests
$242
View
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