About Brilliant Violet 605

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Brilliant Violet™ 605 (BV605) is an orange/red-emitting tandem fluorophore that can be excited by the 405 nm violet laser and collected using a 610/20 bandpass filter. BV605 has an excitation peak at 405 nm and an and emission peak at 603 nm. Due to its unique stoke’s shift, not many dyes have identical excitation and emission spectrums, but the nearest alternatives include SuperBright 600, Qdot™ 605 and eFluor™ 605NC (ThermoFisher). BV605 exhibits a medium level of brightness and is most often used in flow cytometry, specifically, it is an alternative to nanocrystals used for intracellular flow. This dye is part of the Brilliant Violet dye line. The Brilliant Violet dye line itself is very popular because other dyes in the line also use the Brilliant Violet 421 core as the donor molecule, while varied acceptor molecules emit light at various points across the visible light spectrum. This allows for multiple dyes to be compatible while excited by the single laser. In cytometry, a user may populate all the available channels off their 405-violet laser with Brilliant Violet dyes.

Dyes similar to Brilliant Violet 605 based on emission

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AccuOrange Alexa Fluor 568 ATTO RHO101 ATTO Rho11 ATTO Rho12 BOBO-3 BODIPY 581/591 Calcium Crimson (Ca2+bound) California Red CellTracker Red CF575 CF583 CF583R Cardinal Red CyTRAK Orange DY-350XL DY-511XL Dyomics 590 eFluor 605NC EMA eVolve 605 Flash Phalloidin Red 594 GelRed iFluor 594 Janelia Fluor 585 JC-1 KikGR1 (red) Live/Dead Fix Orange Live/Dead Fix Red Live-or-Dye 375/600 Live-or-Dye NucFix Red LSS-mKate2 LysoBrite Red MagDot 610 mCherry MitoLite Red FX600 MitoTracker Red mRFP1 mRuby mStrawberry MultiDot 610 PE-Dazzle 594 PE-Dyomics 590 PE-eFluor 610 PhenoVue 578 Mitochondrial Stain PhenoVue Fluor 568 PROTEOSTAT-Red Qdot 605 ReadiLink 594-610 RPE-CF583R Spark YG 593 STAR 470SXP STAR 580 StarBright UltraViolet 605 StarBright Violet 610 SuperBright 600 SuperNova v605 SureLight PE-594 TFAX 568 SE VivaFix 583/603 X-Rhod-1 (Ca2+ bound) X-Rhod-5F (Ca2+ bound)