Ethidium B.
Ethidium B. (Ex-Max 524 nm/Em-Max 615 nm)
Ethidium Bromide is fluorogenic, cell-impermeant nucleic acid stain with an excitation peak at 524 nm and an emission peak at 615 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 524 nm
- Emission maximum
- 615 nm
- Optimal laser line
- 532
- Optimal filter
- 610/20
Ethidium B. has an excitation maximum at 524 nm and an emission maximum at 615 nm, a Stokes shift of 91 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is Ethidium B.?
- Ethidium Bromide is fluorogenic, cell-impermeant nucleic acid stain with an excitation peak at 524 nm and an emission peak at 615 nm.
- What laser excites Ethidium B.?
- Ethidium B. is optimally excited by the 532 laser line. Its excitation maximum is 524 nm.
- What filter should I use to detect Ethidium B.?
- A 610/20 filter captures the peak of the Ethidium B. emission near 615 nm with minimal spillover.