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X-Rhod-5F (Ca2+ bound)

X-Rhod-5F (Ca2+ bound) (Ex-Max 578 nm/Em-Max 605 nm)

X-Rhod-5F (Ca2+ bound) has an excitation maximum at 578 nm and an emission maximum at 605 nm, a Stokes shift of 27 nm. It is optimally excited by the 561 laser line.

Excitation & emission spectra

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Key facts

Excitation maximum
578 nm
Emission maximum
605 nm
Optimal laser line
561
Optimal filter
610/20

X-Rhod-5F (Ca2+ bound) has an excitation maximum at 578 nm and an emission maximum at 605 nm, a Stokes shift of 27 nm. It is optimally excited by the 561 laser line.

Frequently asked questions

What laser excites X-Rhod-5F (Ca2+ bound)?
X-Rhod-5F (Ca2+ bound) is optimally excited by the 561 laser line. Its excitation maximum is 578 nm.
What filter should I use to detect X-Rhod-5F (Ca2+ bound)?
A 610/20 filter captures the peak of the X-Rhod-5F (Ca2+ bound) emission near 605 nm with minimal spillover.