EvaRuby
EvaRuby (Ex-Max 480 nm/Em-Max 613 nm)
EvaRuby from Biotium is a fluorogenic nucleic acid stain often used for in-gel detection of DNA and qPCR amplification. EvaRuby is compatible with qPCR, HRM and LAMP.
Excitation & emission spectra
Key facts
- Excitation maximum
- 480 nm
- Emission maximum
- 613 nm
- Optimal laser line
- 488
- Optimal filter
- 610/20
EvaRuby has an excitation maximum at 480 nm and an emission maximum at 613 nm, a Stokes shift of 133 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is EvaRuby?
- EvaRuby from Biotium is a fluorogenic nucleic acid stain often used for in-gel detection of DNA and qPCR amplification. EvaRuby is compatible with qPCR, HRM and LAMP.
- What laser excites EvaRuby?
- EvaRuby is optimally excited by the 488 laser line. Its excitation maximum is 480 nm.
- What filter should I use to detect EvaRuby?
- A 610/20 filter captures the peak of the EvaRuby emission near 613 nm with minimal spillover.