LIVE 590
LIVE 590 (Ex-Max 585 nm/Em-Max 609 nm)
abberior LIVE 590 is a member of a new class of fluorescent dyes for live-cell imaging exclusively offered by abberior. This dye is highly photostable and bright orange fluorescent dye, which can be effectively excited with excitation light between 550 - 610 nm. For STED, a depletion wavelength between 750 - 800 nm is recommended. Due to the unique isomeric structure the favorited conformation is stabilized, leading to an enhanced cell permeability of the dye. abberior LIVE 590 can therefore be used in very low nanomolar concentration, reducing background and cytotoxicity effectively, allowing long-term STED and confocal imaging of living cells or tissue.
Excitation & emission spectra
Key facts
- Excitation maximum
- 585 nm
- Emission maximum
- 609 nm
- Optimal laser line
- 561
- Extinction coefficient
- 105000
LIVE 590 has an excitation maximum at 585 nm and an emission maximum at 609 nm, a Stokes shift of 24 nm. Its extinction coefficient is 105000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is LIVE 590?
- abberior LIVE 590 is a member of a new class of fluorescent dyes for live-cell imaging exclusively offered by abberior. This dye is highly photostable and bright orange fluorescent dye, which can be effectively excited with excitation light between 550 - 610 nm. For STED, a depletion wavelength between 750 - 800 nm is recommended. Due to the unique isomeric structure the favorited conformation is stabilized, leading to an enhanced cell permeability of the dye. abberior LIVE 590 can therefore be used in very low nanomolar concentration, reducing background and cytotoxicity effectively, allowing long-term STED and confocal imaging of living cells or tissue.
- What laser excites LIVE 590?
- LIVE 590 is optimally excited by the 561 laser line. Its excitation maximum is 585 nm.