mCherry
mCherry (Ex-Max 584 nm/Em-Max 610 nm)
<html>mCherry is a monomeric fluorescent protein derived from the coral anemones, <i>Discosoma sp. </i>It has an excitation peak at 587 nm and an emission peak at 610 nm.</html>
Excitation & emission spectra
Key facts
- Excitation maximum
- 584 nm
- Emission maximum
- 610 nm
- Optimal laser line
- 594
- Optimal filter
- 610/20
mCherry has an excitation maximum at 584 nm and an emission maximum at 610 nm, a Stokes shift of 26 nm. It is optimally excited by the 594 laser line.
Frequently asked questions
- What is mCherry?
- <html>mCherry is a monomeric fluorescent protein derived from the coral anemones, <i>Discosoma sp. </i>It has an excitation peak at 587 nm and an emission peak at 610 nm.</html>
- What laser excites mCherry?
- mCherry is optimally excited by the 594 laser line. Its excitation maximum is 584 nm.
- What filter should I use to detect mCherry?
- A 610/20 filter captures the peak of the mCherry emission near 610 nm with minimal spillover.