LIVE ORANGE
LIVE ORANGE (Ex-Max 586 nm/Em-Max 606 nm)
abberior LIVE ORANGE mito is a fluorescent probe that specifically stains the cristae in mitochondria. Now, in combination with STED microscopy, these fine mitochondrial components become visible and can be investigate using this organic fluorophore. Due to the chemical structure of the mito-probe, repeated excitation with light over a long period of time is possible without generating toxic effects. This creates the possibility to observe the movement of mitochondria and their substructures through microscopic imaging. The probe can be combined with other fluorescent probes from our abberior LIVE series. This probe shows bright orange fluorescence, which can be effectively excited with a 561 nm laser. For STED, a depletion wavelength between 750 - 800 nm is recommended. This product contains 1 vial of abberior LIVE ORANGE mito as lyophilized powder, a stock solution of 1 mM in DMF or DMSO is recommended. Depending on cell type or tissue a working solution of 100 nM to 500 nM is advised.
Excitation & emission spectra
Key facts
- Excitation maximum
- 586 nm
- Emission maximum
- 606 nm
- Optimal laser line
- 561
- Extinction coefficient
- 115000
LIVE ORANGE has an excitation maximum at 586 nm and an emission maximum at 606 nm, a Stokes shift of 20 nm. Its extinction coefficient is 115000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is LIVE ORANGE?
- abberior LIVE ORANGE mito is a fluorescent probe that specifically stains the cristae in mitochondria. Now, in combination with STED microscopy, these fine mitochondrial components become visible and can be investigate using this organic fluorophore. Due to the chemical structure of the mito-probe, repeated excitation with light over a long period of time is possible without generating toxic effects. This creates the possibility to observe the movement of mitochondria and their substructures through microscopic imaging. The probe can be combined with other fluorescent probes from our abberior LIVE series. This probe shows bright orange fluorescence, which can be effectively excited with a 561 nm laser. For STED, a depletion wavelength between 750 - 800 nm is recommended. This product contains 1 vial of abberior LIVE ORANGE mito as lyophilized powder, a stock solution of 1 mM in DMF or DMSO is recommended. Depending on cell type or tissue a working solution of 100 nM to 500 nM is advised.
- What laser excites LIVE ORANGE?
- LIVE ORANGE is optimally excited by the 561 laser line. Its excitation maximum is 586 nm.