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SureLight PE-594
SureLight PE-594 (Ex-Max 565 nm/Em-Max 610 nm)
SureLight PE-594 from Columbia Biosciences is a tandem with an excitation peak at 488 and 565 nm and an emission peak at 610 nm. SureLight PE-594 is available in antibody conjugation kits for use in flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 565 nm
- Emission maximum
- 610 nm
- Optimal laser line
- 488-561
- Optimal filter
- 610/20
- Relative brightness
- 4 of 5
SureLight PE-594 has an excitation maximum at 565 nm and an emission maximum at 610 nm, a Stokes shift of 45 nm. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is SureLight PE-594?
- SureLight PE-594 from Columbia Biosciences is a tandem with an excitation peak at 488 and 565 nm and an emission peak at 610 nm. SureLight PE-594 is available in antibody conjugation kits for use in flow cytometry.
- What laser excites SureLight PE-594?
- SureLight PE-594 is optimally excited by the 488-561 laser line. Its excitation maximum is 565 nm.
- What filter should I use to detect SureLight PE-594?
- A 610/20 filter captures the peak of the SureLight PE-594 emission near 610 nm with minimal spillover.
- How bright is SureLight PE-594?
- SureLight PE-594 has a relative brightness of 4 out of 5.