RPE-CF583R
RPE-CF®583R (Ex-Max 565 nm/Em-Max 605 nm)
RPE-CF®583R is a tandem fluorophore between R-PE (donor) and CF®583R (acceptor) that can be excited off the 488, 532 or 561 nm laser and has an emission peak at 608 nm. It can be used with the following analysis platforms: Spectral Flow Cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 565 nm
- Emission maximum
- 605 nm
- Optimal laser line
- 488-561
- Optimal filter
- 610/20
- Relative brightness
- 4 of 5
RPE-CF583R has an excitation maximum at 565 nm and an emission maximum at 605 nm, a Stokes shift of 40 nm. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is RPE-CF583R?
- RPE-CF®583R is a tandem fluorophore between R-PE (donor) and CF®583R (acceptor) that can be excited off the 488, 532 or 561 nm laser and has an emission peak at 608 nm. It can be used with the following analysis platforms: Spectral Flow Cytometry.
- What laser excites RPE-CF583R?
- RPE-CF583R is optimally excited by the 488-561 laser line. Its excitation maximum is 565 nm.
- What filter should I use to detect RPE-CF583R?
- A 610/20 filter captures the peak of the RPE-CF583R emission near 605 nm with minimal spillover.
- How bright is RPE-CF583R?
- RPE-CF583R has a relative brightness of 4 out of 5.