VivaFix 583/603
VivaFix 583/603 (Ex-Max 583 nm/Em-Max 603 nm)
VivaFix 583/603 from Bio-Rad is a fixable viability dye useful in flow cytometry in applications where the cells will be fixed and permeabilized. It has an excitation peak at 583 nm and an emission peak at 603 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 583 nm
- Emission maximum
- 603 nm
- Optimal laser line
- 561
- Optimal filter
- 610/20
VivaFix 583/603 has an excitation maximum at 583 nm and an emission maximum at 603 nm, a Stokes shift of 20 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is VivaFix 583/603?
- VivaFix 583/603 from Bio-Rad is a fixable viability dye useful in flow cytometry in applications where the cells will be fixed and permeabilized. It has an excitation peak at 583 nm and an emission peak at 603 nm.
- What laser excites VivaFix 583/603?
- VivaFix 583/603 is optimally excited by the 561 laser line. Its excitation maximum is 583 nm.
- What filter should I use to detect VivaFix 583/603?
- A 610/20 filter captures the peak of the VivaFix 583/603 emission near 603 nm with minimal spillover.