Live-or-Dye 594/614
Live-or-Dye™ 594/614 (Ex-Max 593 nm/Em-Max 615 nm)
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 594/614 has an excitation peak at 594 nm and an emission peak at 614 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 593 nm
- Emission maximum
- 615 nm
- Optimal laser line
- 488-561
- Optimal filter
- 610/20
- Commercial conjugates
- 2 from 1 supplier
Live-or-Dye 594/614 has an excitation maximum at 593 nm and an emission maximum at 615 nm, a Stokes shift of 22 nm. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is Live-or-Dye 594/614?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 594/614 has an excitation peak at 594 nm and an emission peak at 614 nm.
- What laser excites Live-or-Dye 594/614?
- Live-or-Dye 594/614 is optimally excited by the 488-561 laser line. Its excitation maximum is 593 nm.
- What filter should I use to detect Live-or-Dye 594/614?
- A 610/20 filter captures the peak of the Live-or-Dye 594/614 emission near 615 nm with minimal spillover.
- How many antibodies are available conjugated to Live-or-Dye 594/614?
- FluoroFinder tracks 2 commercially available Live-or-Dye 594/614 conjugates, searchable by target, clone, host and application.