Dyes similar to MemBrite Fix 594/615 based on emissionAlexa Fluor 594 Alexa Fluor 610 ATTO RHO101 BDY TR-X SE BODIPY TR BUV615 Calcium Crimson (Ca2+bound) CF583 CF583R CF594 CF594ST CF597R CoraLite 594 CoraLite +595 CyTRAK Orange DY-350XL DyLight 594 ECD eFluor 615 Ethidium B. Ethidium Homodimer I EvaRuby Flash Phalloidin Red 594 FVS620 Hc Red HiLyte 594 iFluor 594 Janelia Fluor 585 Live/Dead Fix Red Live-or-Dye 594/614 LSS-mKate1 LysoBrite Deep Red MagDot 610 Magic Red mCherry mKeima mRaspberry mRFP1 MultiDot 610 NovaFluor Blue 610 / 30S NovaFluor Blue 610 / 70S NovaFluor Yellow 610 PE-CF594 PE-Dazzle 594 PE-DyLight 594 PE-eFluor 610 PE-Texas Red PE-Vio615 PhenoVue Fluor 594 PI PMA PMAxx PromoFluor 594 Protoporphyrin IX ReadiLink 594-610 STAR 470SXP STAR 520SXP STAR 580 STAR 600 StarBright UltraViolet 605 StarBright Violet 610 STAR ORANGE SuperNova v605 SureLight PE-594 Texas Red TFAX 594 SE Zombie Red
About MemBrite Fix 594/615
Biotium’s MemBrite Fix and Membrite Fix-ST dyes are cell surface staining dyes that irreversibly react with cell surface proteins to stain the outer membrane. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and imaging techniques such as STORM. This dye line shares similarities with Biotium’s CellBrite line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.