Loading the Dye Directory…

BODIPY R6G

BODIPY R6G (Ex-Max 526 nm/Em-Max 544 nm)

BODIPY R6G is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 526 nm and an emission peak at 544 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.

Excitation & emission spectra

400450500550600650700750800

Key facts

Excitation maximum
526 nm
Emission maximum
544 nm
Optimal laser line
532
Optimal filter
525/50
Molecular weight
437.21

BODIPY R6G has an excitation maximum at 526 nm and an emission maximum at 544 nm, a Stokes shift of 18 nm. It is optimally excited by the 532 laser line.

Frequently asked questions

What is BODIPY R6G?
BODIPY R6G is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 526 nm and an emission peak at 544 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.
What laser excites BODIPY R6G?
BODIPY R6G is optimally excited by the 532 laser line. Its excitation maximum is 526 nm.
What filter should I use to detect BODIPY R6G?
A 525/50 filter captures the peak of the BODIPY R6G emission near 544 nm with minimal spillover.