LSS-mKate1
LSS-mKate1 (Ex-Max 463 nm/Em-Max 624 nm)
LSS-mKate1 is a monomeric fluorescent protein derived from the sea anemones, Entacmaea, quadricolor. It has an excitation peak at 463 nm and an emission peak at 624 nm. It has a wide excitation profile and can be efficiently excited between 405-488 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 463 nm
- Emission maximum
- 624 nm
- Optimal laser line
- 488
- Optimal filter
- 630/69
- Extinction coefficient
- 31200
- Quantum yield
- 0.08
- Molecular weight
- 26200
LSS-mKate1 has an excitation maximum at 463 nm and an emission maximum at 624 nm, a Stokes shift of 161 nm. Its extinction coefficient is 31200 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is LSS-mKate1?
- LSS-mKate1 is a monomeric fluorescent protein derived from the sea anemones, Entacmaea, quadricolor. It has an excitation peak at 463 nm and an emission peak at 624 nm. It has a wide excitation profile and can be efficiently excited between 405-488 nm.
- What laser excites LSS-mKate1?
- LSS-mKate1 is optimally excited by the 488 laser line. Its excitation maximum is 463 nm.
- What filter should I use to detect LSS-mKate1?
- A 630/69 filter captures the peak of the LSS-mKate1 emission near 624 nm with minimal spillover.