ATTO 590
ATTO 590 from ATTO-TEC is a novel fluorescent label belonging to the class of rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Thus ATTO 590 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The dye is moderately hydrophilic. As supplied ATTO 590 consists of a mixture of two isomers with practically identical absorption and fluorescence.
Excitation & emission spectra
Key facts
- Excitation maximum
- 593 nm
- Emission maximum
- 622 nm
- Optimal laser line
- 561, 594
- Relative brightness
- 2 of 5
- Extinction coefficient
- 120000
- Quantum yield
- .80
- Fluorescence lifetime
- 3.7 ns
- Commercial antibodies
- 3 from 1 supplier
ATTO 590 has an excitation maximum at 593 nm and an emission maximum at 622 nm, a Stokes shift of 29 nm. Its extinction coefficient is 120000 M⁻¹cm⁻¹. It is optimally excited by the 561, 594 laser line.
Frequently asked questions
- What is ATTO 590?
- ATTO 590 from ATTO-TEC is a novel fluorescent label belonging to the class of rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Thus ATTO 590 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The dye is moderately hydrophilic. As supplied ATTO 590 consists of a mixture of two isomers with practically identical absorption and fluorescence.
- What laser excites ATTO 590?
- ATTO 590 is optimally excited by the 561, 594 laser line. Its excitation maximum is 593 nm.
- How bright is ATTO 590?
- ATTO 590 has a relative brightness of 2 out of 5 (quantum yield .80).
- How many antibodies are available conjugated to ATTO 590?
- FluoroFinder tracks 3 commercially available ATTO 590 conjugates, searchable by target, clone, host and application.