Ethidium Homodimer I
Ethidium Homodimer I (Ex-Max 527 nm/Em-Max 624 nm)
Ethidium Homodimer I (EthD-1) is a cell-impermeant nucleic acid stain with an excitation peak at 527 nm and an emission peak 625 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 527 nm
- Emission maximum
- 624 nm
- Optimal laser line
- 532
- Optimal filter
- 630/69
Ethidium Homodimer I has an excitation maximum at 527 nm and an emission maximum at 624 nm, a Stokes shift of 97 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is Ethidium Homodimer I?
- Ethidium Homodimer I (EthD-1) is a cell-impermeant nucleic acid stain with an excitation peak at 527 nm and an emission peak 625 nm.
- What laser excites Ethidium Homodimer I?
- Ethidium Homodimer I is optimally excited by the 532 laser line. Its excitation maximum is 527 nm.
- What filter should I use to detect Ethidium Homodimer I?
- A 630/69 filter captures the peak of the Ethidium Homodimer I emission near 624 nm with minimal spillover.