Protoporphyrin IX
Protoporphyrin IX (Ex-Max 393 nm/Em-Max 621 nm)
Protoporphyrin IX is a naturally occurring autofluorescent compound involved in heme biosynthesis. It's tetrapyrrole structure enables it to chelate transitional metals. Specifically, chelation of PPIX with iron forms heme.
Excitation & emission spectra
Key facts
- Excitation maximum
- 393 nm
- Emission maximum
- 621 nm
- Optimal laser line
- 405
- Optimal filter
- 610/20
- Relative brightness
- 1 of 5
- Commercial antibodies
- 1 from 1 supplier
Protoporphyrin IX has an excitation maximum at 393 nm and an emission maximum at 621 nm, a Stokes shift of 228 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Protoporphyrin IX?
- Protoporphyrin IX is a naturally occurring autofluorescent compound involved in heme biosynthesis. It's tetrapyrrole structure enables it to chelate transitional metals. Specifically, chelation of PPIX with iron forms heme.
- What laser excites Protoporphyrin IX?
- Protoporphyrin IX is optimally excited by the 405 laser line. Its excitation maximum is 393 nm.
- What filter should I use to detect Protoporphyrin IX?
- A 610/20 filter captures the peak of the Protoporphyrin IX emission near 621 nm with minimal spillover.
- How bright is Protoporphyrin IX?
- Protoporphyrin IX has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to Protoporphyrin IX?
- FluoroFinder tracks 1 commercially available Protoporphyrin IX conjugates, searchable by target, clone, host and application.