PMA
PMA (Ex-Max 530 nm/Em-Max 622 nm)
PMA (Propidium Monoazide) is a fluorogenic, cell-impermeant nucleic acid stain that upon photo-activation, can crosslink to DNA for increased retention of the signal. It is commonly used for Real Time PCR viability assessment in bacteria, viruses and fungi. It has an excitation peak at 530 nm and an emission peak at 622 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 530 nm
- Emission maximum
- 622 nm
- Optimal laser line
- 488-532
- Optimal filter
- 610/20
PMA has an excitation maximum at 530 nm and an emission maximum at 622 nm, a Stokes shift of 92 nm. It is optimally excited by the 488-532 laser line.
Frequently asked questions
- What is PMA?
- PMA (Propidium Monoazide) is a fluorogenic, cell-impermeant nucleic acid stain that upon photo-activation, can crosslink to DNA for increased retention of the signal. It is commonly used for Real Time PCR viability assessment in bacteria, viruses and fungi. It has an excitation peak at 530 nm and an emission peak at 622 nm.
- What laser excites PMA?
- PMA is optimally excited by the 488-532 laser line. Its excitation maximum is 530 nm.
- What filter should I use to detect PMA?
- A 610/20 filter captures the peak of the PMA emission near 622 nm with minimal spillover.