NADH
NADH (Ex-Max 337 nm/Em-Max 464 nm)
NADH, known as nicotinamide adenine dinucleotide + hydrogen, is a naturally ocurring compound in the body that plays a key role in energy production and contributes to the autofluorescent background in most cell and tissue preparations.
Excitation & emission spectra
Key facts
- Excitation maximum
- 337 nm
- Emission maximum
- 464 nm
- Optimal laser line
- 320-405
- Optimal filter
- 450/50
NADH has an excitation maximum at 337 nm and an emission maximum at 464 nm, a Stokes shift of 127 nm. It is optimally excited by the 320-405 laser line.
Frequently asked questions
- What is NADH?
- NADH, known as nicotinamide adenine dinucleotide + hydrogen, is a naturally ocurring compound in the body that plays a key role in energy production and contributes to the autofluorescent background in most cell and tissue preparations.
- What laser excites NADH?
- NADH is optimally excited by the 320-405 laser line. Its excitation maximum is 337 nm.
- What filter should I use to detect NADH?
- A 450/50 filter captures the peak of the NADH emission near 464 nm with minimal spillover.