ATTO Rho13
ATTO Rho13 (Ex-Max 600 nm/Em-Max 625 nm)
ATTO Rho13 from ATTO-TEC has an excitation peak at 600 nm and an emission peak at 625 nm. ATTO Rho13 is a new fluorescent label belonging to the class of rhodamines. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. ATTO Rho13 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. The label is moderately hydrophilic.
Excitation & emission spectra
Key facts
- Excitation maximum
- 600 nm
- Emission maximum
- 625 nm
- Optimal laser line
- 561, 594
- Optimal filter
- 610/20
- Relative brightness
- 2 of 5
- Extinction coefficient
- 120000
- Quantum yield
- 0.8
- Fluorescence lifetime
- 3.9 ns
- Molecular weight
- 770
ATTO Rho13 has an excitation maximum at 600 nm and an emission maximum at 625 nm, a Stokes shift of 25 nm. Its extinction coefficient is 120000 M⁻¹cm⁻¹. It is optimally excited by the 561, 594 laser line.
Frequently asked questions
- What is ATTO Rho13?
- ATTO Rho13 from ATTO-TEC has an excitation peak at 600 nm and an emission peak at 625 nm. ATTO Rho13 is a new fluorescent label belonging to the class of rhodamines. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. ATTO Rho13 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. The label is moderately hydrophilic.
- What laser excites ATTO Rho13?
- ATTO Rho13 is optimally excited by the 561, 594 laser line. Its excitation maximum is 600 nm.
- What filter should I use to detect ATTO Rho13?
- A 610/20 filter captures the peak of the ATTO Rho13 emission near 625 nm with minimal spillover.
- How bright is ATTO Rho13?
- ATTO Rho13 has a relative brightness of 2 out of 5 (quantum yield 0.8).