Chromeo 494 Dye Profile
488
Laser Line
630/69
Common Filter
494
Excitation Max
628
Emission Max
Dyes similar to Chromeo 494 based on emission
Alexa Fluor 610 ATTO 594 ATTO 610 ATTO Rho13 BB630-P2 BODIPY TR CF597R DY-480XL DY-601XL ECD eFluor 625NC Ethidium Homodimer I Ethidium Homodimer III Fura Red AM (Ca2+bound) Katushka2S Live-or-Dye 640/662 LSS-mKate1 LysoView 540 LysoView 594 Magic Red mKate2 mKate mKeima mRaspberry Oxazole Red Oxazole Red Homodimer PE-Alexa 610 PE-ATTO 594 PE-Vio615 PhenoVue Nile Red Lipid Stain PMA PMAxx Protoporphyrin IX Qdot 625 STAR 600 SureLight RPC YO-PRO-3 YOYO-3 Zombie RedAbout Chromeo 494
Chromeo™ 494 is a unique red fluorescent label that is excitable at a wavelength of 488 nm by Argon Laser, LED, Xenon-Arc or Tungsten-Halogen lamps. It has a very large Stokes shift of 134 nm, making it an ideal partner for multiplexing in immunofluorescence experiments, especially in combination with Chromeo 488. While both dyes are readily excited at 488 nm, the fluorescent emission of Chromeo 488 at 517 nm is easily distinguished from the emission of Chromeo 494 at 628 nm. This long Stokes shift eliminates signal overlap caused by bleed-through between the detection channels in multiplexing experiments.
