Ethidium Homodimer III
Ethidium Homodimer III (Ex-Max 531 nm/Em-Max 625 nm)
Ethidium Homodimer II (Eth-D2) is a cell-impermeant nucleic acid stain with an excitation peak at 531 nm and an emission peak 625 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 531 nm
- Emission maximum
- 625 nm
- Optimal laser line
- 532
- Optimal filter
- 630/69
Ethidium Homodimer III has an excitation maximum at 531 nm and an emission maximum at 625 nm, a Stokes shift of 94 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is Ethidium Homodimer III?
- Ethidium Homodimer II (Eth-D2) is a cell-impermeant nucleic acid stain with an excitation peak at 531 nm and an emission peak 625 nm.
- What laser excites Ethidium Homodimer III?
- Ethidium Homodimer III is optimally excited by the 532 laser line. Its excitation maximum is 531 nm.
- What filter should I use to detect Ethidium Homodimer III?
- A 630/69 filter captures the peak of the Ethidium Homodimer III emission near 625 nm with minimal spillover.