LysoSensor Yellow/Blue pH3.0
LysoSensor Yellow/Blue pH3.0 (Ex-Max 388 nm/Em-Max 540 nm)
LysoSensor Yellow/Blue is a ratiometric probe that measures the pH of acidic lysosomes and endosomes. The pKa of LysoSensor Yellow/Blue DND-160 is ~4.2. Because of its unique spectral characteristics in that both the excitation and emission profiles change, this probe is used primarily in live-cell imaging.
Excitation & emission spectra
Key facts
- Excitation maximum
- 384 nm
- Emission maximum
- 540 nm
- Optimal laser line
- 355
- Optimal filter
- 525/50
LysoSensor Yellow/Blue pH3.0 has an excitation maximum at 384 nm and an emission maximum at 540 nm, a Stokes shift of 156 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is LysoSensor Yellow/Blue pH3.0?
- LysoSensor Yellow/Blue is a ratiometric probe that measures the pH of acidic lysosomes and endosomes. The pKa of LysoSensor Yellow/Blue DND-160 is ~4.2. Because of its unique spectral characteristics in that both the excitation and emission profiles change, this probe is used primarily in live-cell imaging.
- What laser excites LysoSensor Yellow/Blue pH3.0?
- LysoSensor Yellow/Blue pH3.0 is optimally excited by the 355 laser line. Its excitation maximum is 384 nm.
- What filter should I use to detect LysoSensor Yellow/Blue pH3.0?
- A 525/50 filter captures the peak of the LysoSensor Yellow/Blue pH3.0 emission near 540 nm with minimal spillover.