Janelia Fluor 669
Janelia Fluor® 669 (Ex-Max 669 nm/Em-Max 682 nm)
Janelia Fluor® 669 was developed at the Janelia Campus of the Howard Hughes Medical Institute but is commercialized by other vendors. The Janelia Fluor®s family is unique in that the fluorophores are cell-permeable and are available in photoactivatable forms. These fluorophores were developed for super-resolution microscopy (STED, PALM and STORM) and live-cell microscopy in the HaloTag and SNAP-tag versions. Janelia Fluor® 669 has an excitation peak at 669 nm and an emission peak at 682 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 669 nm
- Emission maximum
- 682 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Relative brightness
- 3 of 5
- Extinction coefficient
- 116000
- Quantum yield
- 0.37
Janelia Fluor 669 has an excitation maximum at 669 nm and an emission maximum at 682 nm, a Stokes shift of 13 nm. Its extinction coefficient is 116000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Janelia Fluor 669?
- Janelia Fluor® 669 was developed at the Janelia Campus of the Howard Hughes Medical Institute but is commercialized by other vendors. The Janelia Fluor®s family is unique in that the fluorophores are cell-permeable and are available in photoactivatable forms. These fluorophores were developed for super-resolution microscopy (STED, PALM and STORM) and live-cell microscopy in the HaloTag and SNAP-tag versions. Janelia Fluor® 669 has an excitation peak at 669 nm and an emission peak at 682 nm.
- What laser excites Janelia Fluor 669?
- Janelia Fluor 669 is optimally excited by the 628-640 laser line. Its excitation maximum is 669 nm.
- What filter should I use to detect Janelia Fluor 669?
- A 695/40 filter captures the peak of the Janelia Fluor 669 emission near 682 nm with minimal spillover.
- How bright is Janelia Fluor 669?
- Janelia Fluor 669 has a relative brightness of 3 out of 5 (quantum yield 0.37).