About MemBrite Fix 660/680

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Biotium’s MemBrite Fix and Membrite Fix-ST dyes are cell surface staining dyes that irreversibly react with cell surface proteins to stain the outer membrane. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and imaging techniques such as STORM. This dye line shares similarities with Biotium’s CellBrite line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.

Dyes similar to MemBrite Fix 660/680 based on emission

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Alexa Fluor 660 ATTO 655 ATTO 665 BactoView Live Red CellBrite Steady 650 CellMask Deep Red Plasma Membrane Stain CellVue Burgundy CF660C CF660R CF660ST CPN680 Red DRAQ 5 ER-ID Red HiLyte 647 iRFP 682 Janelia Fluor 669 Live-or-Dye 665/685 LysoView 650 MaxLight 650 MemBrite Fix ST 667/685 MITO-ID Red MitoLite NIR FX690 NovaFluor Red 685 NovaFluor Yellow 690 Nuclear Red NucSpot Live 650 PerCP PromoFluor 647P ReadiLink 647-674 RPE-CF647T Seta-PerCP-680 Spark NIR 685 SureLight PerCP Sytox Deep Red ViaFluor 647 Vybrant DyeCycle Ruby