Dyes similar to MemBrite Fix ST 667/685 based on emissionAlexa Fluor 660 ATTO 655 ATTO 665 CellMask Deep Red Plasma Membrane Stain CellVue Burgundy CF660C CF660R CF660ST DRAQ 5 ER-ID Red IRDye 680LT IRDye 680RD iRFP 682 Janelia Fluor 669 Live-or-Dye 665/685 LysoView 650 MaxLight 650 MemBrite Fix 660/680 MITO-ID Red MitoLite NIR FX690 NovaFluor Red 685 NovaFluor Yellow 690 Nuclear Red NucSpot Live 650 Oyster 680 RedDot 1 Seta-PerCP-680 SeTau-647-di-NHS SeTau-670-NHS Spark NIR 685 SureLight PerCP Sytox Deep Red ViaFluor 647 Vybrant DyeCycle Ruby
About MemBrite Fix ST 667/685
Biotium’s MemBrite Fix and Membrite Fix-ST dyes are cell surface staining dyes that irreversibly react with cell surface proteins to stain the outer membrane. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and imaging techniques such as STORM. This dye line shares similarities with Biotium’s CellBrite line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.