DRAQ 5
DRAQ 5 (Ex-Max 647 nm/Em-Max 681 nm)
DRAQ5 from Biostatus is a cell-permeant, fluorogenic nucleic acid stain that has an excitation peak at 647 nm and an emission peak at 681 nm when intercalated with dsDNA. DRAQ5 has a high-affinity for dsDNA and is used to counterstain live or fixed cell nuclei. It binds DNA stoichiometrically so can be used to quantitate nuclear DNA content for ploidy or cell cycle analysis. DRAQ dyes are manufactured and sold by Biostatus.
Excitation & emission spectra
Key facts
- Excitation maximum
- 647 nm
- Emission maximum
- 681 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Extinction coefficient
- 22000
- Molecular weight
- 413
- Commercial antibodies
- 6 from 3 suppliers
DRAQ 5 has an excitation maximum at 647 nm and an emission maximum at 681 nm, a Stokes shift of 34 nm. Its extinction coefficient is 22000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is DRAQ 5?
- DRAQ5 from Biostatus is a cell-permeant, fluorogenic nucleic acid stain that has an excitation peak at 647 nm and an emission peak at 681 nm when intercalated with dsDNA. DRAQ5 has a high-affinity for dsDNA and is used to counterstain live or fixed cell nuclei. It binds DNA stoichiometrically so can be used to quantitate nuclear DNA content for ploidy or cell cycle analysis. DRAQ dyes are manufactured and sold by Biostatus.
- What laser excites DRAQ 5?
- DRAQ 5 is optimally excited by the 628-640 laser line. Its excitation maximum is 647 nm.
- What filter should I use to detect DRAQ 5?
- A 695/40 filter captures the peak of the DRAQ 5 emission near 681 nm with minimal spillover.
- How many antibodies are available conjugated to DRAQ 5?
- FluoroFinder tracks 6 commercially available DRAQ 5 conjugates, searchable by target, clone, host and application.