Spark NIR 685
Spark NIR 685 (Ex-Max 660 nm/Em-Max 685 nm)
Spark NIR® 685 from Biolegend is a fluorophore designed for spectral flow cytometry with an excitation peak at 633 nm and an emission peak at 685 nm. This fluorophore is an alternative to Alexa Fluor™ 660 and can be efficiently excited by the 633 nm laser and can be unmixed from Alexa Fluor™ 647 and Alexa Fluor™ 700.
Excitation & emission spectra
Key facts
- Excitation maximum
- 660 nm
- Emission maximum
- 685 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Relative brightness
- 2 of 5
- Molecular weight
- 3100
- Commercial antibodies
- 296 from 2 suppliers
Spark NIR 685 has an excitation maximum at 660 nm and an emission maximum at 685 nm, a Stokes shift of 25 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Spark NIR 685?
- Spark NIR® 685 from Biolegend is a fluorophore designed for spectral flow cytometry with an excitation peak at 633 nm and an emission peak at 685 nm. This fluorophore is an alternative to Alexa Fluor™ 660 and can be efficiently excited by the 633 nm laser and can be unmixed from Alexa Fluor™ 647 and Alexa Fluor™ 700.
- What laser excites Spark NIR 685?
- Spark NIR 685 is optimally excited by the 628-640 laser line. Its excitation maximum is 660 nm.
- What filter should I use to detect Spark NIR 685?
- A 695/40 filter captures the peak of the Spark NIR 685 emission near 685 nm with minimal spillover.
- How bright is Spark NIR 685?
- Spark NIR 685 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to Spark NIR 685?
- FluoroFinder tracks 296 commercially available Spark NIR 685 conjugates, searchable by target, clone, host and application.