NucSpot Live 650 Dye Profile


640

Laser Line

695/40

Common Filter

655

Excitation Max

681

Emission Max


About NucSpot Live 650


Biotium’s NucSpot Live Cell Nuclear Stains are cell-membrane permeant DNA dyes for staining nuclei in live or fixed cells. These dyes exhibit low toxicity and their high specificity for DNA allows for imaging without a wash step. Dyes in the NucSpot Live family include NucSpot Live 488 and NucSpot Live 650. The more general NucSpot family of dyes has a few more members that have different functions compared to these NucSpot Live dyes. The NucSpot 470 dye is cell membrane-impermeant and can only stain nuclei in fixed cells or dead cells, while NucSpot Far-Red is an alternative to 7-AAD and is similarly used for staining cells with compromised cellular membranes. Check out the other members of this dye line in FluoroFinder’s Dye Directory or on our Spectra Viewer.



Spectra