DiR
DiR (Ex-Max 750 nm/Em-Max 782 nm)
DiR is a lipophilic carbocyanine dye often used in fluorescence microscopy to label the cell surface, trace neuronal axons or, upon fixation, any lipid bound compartment. This probe has an excitation peak at 750 nm and an emission peak at 782 nm
Excitation & emission spectra
Key facts
- Excitation maximum
- 750 nm
- Emission maximum
- 782 nm
- Optimal laser line
- 750
- Optimal filter
- 780/60
- Extinction coefficient
- 270000
- Molecular weight
- 1013
DiR has an excitation maximum at 750 nm and an emission maximum at 782 nm, a Stokes shift of 32 nm. Its extinction coefficient is 270000 M⁻¹cm⁻¹. It is optimally excited by the 750 laser line.
Frequently asked questions
- What is DiR?
- DiR is a lipophilic carbocyanine dye often used in fluorescence microscopy to label the cell surface, trace neuronal axons or, upon fixation, any lipid bound compartment. This probe has an excitation peak at 750 nm and an emission peak at 782 nm
- What laser excites DiR?
- DiR is optimally excited by the 750 laser line. Its excitation maximum is 750 nm.
- What filter should I use to detect DiR?
- A 780/60 filter captures the peak of the DiR emission near 782 nm with minimal spillover.