About Brilliant Violet 785

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Brilliant Violet™ 785 (BV785) is a far-red-emitting tandem fluorophore that can be excited by the 405 nm violet laser and collected using a 780/60 bandpass filter. BV785 has an excitation peak at 405 nm and an emission peak at 785 nm. Due to its unique stoke’s shift, few dyes have identical or near identical excitation and emission spectrums, but the nearest alternatives include Qdot™ 800 and SuperBright 780 (ThermoFisher), and Brilliant Violet 786 (BD Biosciences). BV785 exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large stoke’s shift, however BV785 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant Violet dye line. The Brilliant Violet dye line itself is very popular because other dyes in the line also use the Brilliant Violet 421 core as the donor molecule, while varied acceptor molecules emit light at various points across the visible light spectrum. This allows for multiple dyes to be compatible while excited by the single laser. In cytometry, a user may populate all the available channels off their 405-violet laser with Brilliant Violet dyes.

Dyes similar to Brilliant Violet 785 based on emission

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APC-CF750 APC-CF750T APC-Cy7 APC-eFluor 780 APC-Fire 750 APC-H7 Brilliant Blue 790-P Brilliant Violet 786 CellBrite NIR750 CF750 CF754 CF770T Cy7 DiR DyLight 750 DyLight 755 DyLight 800 eFluor 780 Fix Viability Ghost Dye Red 780 HiLyte 750 iFluor 750 IRDye 800CW Live/Dead Fix Near IR (780) Live-or-Dye 750/777 MaxLight 750 MemBrite Fix ST 755/777 PE-Cy7 ReadiLink 750-780 Seta-750-NHS Seta-APC-780 StarBright UltraViolet 795 StarBright Violet 790 SuperBright 780 SuperNova v786 SureLight PE Cyanine 7.0