405

Laser Line

780/60

Common Filter

405

Excitation Max

785

Emission Max

539

Antibodies

1

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About Brilliant Violet 785


Brilliant Violet™ 785 (BV785) is a far-red-emitting tandem fluorophore that can be excited by the 405 nm violet laser and collected using a 780/60 bandpass filter. BV785 has an excitation peak at 405 nm and an emission peak at 785 nm. Due to its unique stoke’s shift, few dyes have identical or near identical excitation and emission spectrums, but the nearest alternatives include Qdot™ 800 and SuperBright 780 (ThermoFisher), and Brilliant Violet 786 (BD Biosciences). BV785 exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large stoke’s shift, however BV785 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant Violet dye line. The Brilliant Violet dye line itself is very popular because other dyes in the line also use the Brilliant Violet 421 core as the donor molecule, while varied acceptor molecules emit light at various points across the visible light spectrum. This allows for multiple dyes to be compatible while excited by the single laser. In cytometry, a user may populate all the available channels off their 405-violet laser with Brilliant Violet dyes.


Popular Brilliant Violet 785 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BioLegend
123141
FC F4/80
Mouse
Rat
IgG2a κ
BM8
50 μg
$275
View
BioLegend
135225
FC PD-1
Mouse
Rat
IgG2a κ
29F.1A12
50 μg
$292
View
BioLegend
304140
FC CD45RA
Human,Chimpanzee
Mouse
IgG2b κ
HI100
100 Tests
$435
View
BioLegend
101243
FC CD11b
Mouse,Human,Chimpanzee,Baboon,Cynomolgus,Rhesus,Rabbit
Rat
IgG2b κ
M1/70
50 μg
$286
View
BioLegend
100232
FC CD3
Mouse
Rat
IgG2b κ
17A2
50 μg
$275
View
BioLegend
317330
FC CD3
Human
Mouse
IgG2a κ
OKT3
100 Tests
$418
View
BioLegend
103149
FC CD45
Mouse
Rat
IgG2b κ
30-F11
50 μg
$286
View
BioLegend
128041
FC Ly-6C
Mouse
Rat
IgG2c κ
HK1.4
50 μg
$286
View
BioLegend
127645
FC Ly-6G
Mouse
Rat
IgG2a κ
1A8
50 μg
$275
View
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