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SureLight PE Cyanine 7.0

SureLight PE-777 (Ex-Max 565 nm/Em-Max 777 nm)

SureLight PE-777 (PE-Cyanine 7) from Columbia Biosciences is a tandem with an excitation peak at 488 and 565 nm and an emission peak at 777 nm. SureLight PE-777 is available in antibody conjugation kits for use in flow cytometry.

Excitation & emission spectra

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Key facts

Excitation maximum
565 nm
Emission maximum
777 nm
Optimal laser line
488-561
Optimal filter
780/60
Relative brightness
4 of 5

SureLight PE Cyanine 7.0 has an excitation maximum at 565 nm and an emission maximum at 777 nm, a Stokes shift of 212 nm. It is optimally excited by the 488-561 laser line.

Frequently asked questions

What is SureLight PE Cyanine 7.0?
SureLight PE-777 (PE-Cyanine 7) from Columbia Biosciences is a tandem with an excitation peak at 488 and 565 nm and an emission peak at 777 nm. SureLight PE-777 is available in antibody conjugation kits for use in flow cytometry.
What laser excites SureLight PE Cyanine 7.0?
SureLight PE Cyanine 7.0 is optimally excited by the 488-561 laser line. Its excitation maximum is 565 nm.
What filter should I use to detect SureLight PE Cyanine 7.0?
A 780/60 filter captures the peak of the SureLight PE Cyanine 7.0 emission near 777 nm with minimal spillover.
How bright is SureLight PE Cyanine 7.0?
SureLight PE Cyanine 7.0 has a relative brightness of 4 out of 5.