Brilliant Violet 786 Dye Profile


405

Laser Line

780/60

Common Filter

405

Excitation Max

786

Emission Max


About Brilliant Violet 786


Brilliant Violet™ 786 (BV786) is a far-red-emitting tandem fluorophore that can be excited by the 405 nm violet laser and collected using a 780/60 bandpass filter. BV750 has an excitation peak at 405 nm and an emission peak at 786 nm. Due to its unique stoke’s shift, few dyes have identical or near identical excitation and emission spectrums, but the nearest alternatives are Qdot™ 800 and SuperBright 780 (ThermoFisher), and Brilliant Violet 785 (BioLegend). What's the difference between BV785 and BV786? BV785 is a BioLegend color, while BV786 is a BD Biosciences color, but they exhibit the same spectral properties. This dye exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large stoke’s shift, however BV786 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant Violet dye line. The Brilliant Violet dye line itself is very popular because other dyes in the line also use the Brilliant Violet 421 core as the donor molecule, while varied acceptor molecules emit light at various points across the visible light spectrum. This allows for multiple dyes to be compatible while excited by the single laser. In cytometry, a user may populate all the available channels off their 405-violet laser with Brilliant Violet dyes.



Spectra


 

Popular Brilliant Violet 786 Secondary Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BD Biosciences
563618
FC IgD
Mouse
Rat
IgG2a κ
11-26c.2a
50 μg
$255
View
BD Biosciences
564230
FC IgG
Human
Mouse
IgG1 κ
G18-145
50 Tests
$285
View
BD Biosciences
740998
FC IgM
Human
Mouse
IgG1 κ
G20-127
50 μg
$495
View
BD Biosciences
740997
FC IgD
Human
Mouse
IgG2a κ
IA6-2
50 μg
$495
View
BD Biosciences
744673
FC IgG
Hamster
Mouse
IgG1 κ
G192-1
50 μg
View
BD Biosciences
742480
FC IgG1
Mouse
Rat
IgG1 κ
X56
50 μg
View
BD Biosciences
743328
FC IgM
Mouse
Rat
IgG2a κ
II/41
50 μg
$350
View
BD Biosciences
564028
FC IgM
Mouse
Rat
IgG2a κ
R6-60.2
50 μg
$265
View
BD Biosciences
742838
FC Ig, k light chain
Mouse
Rat
IgG1 κ
187.1
50 μg
View
BD Biosciences
564206
FC IgE
Mouse
Rat
IgG1 κ
R35-72
50 μg
View
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