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DyLight 755

DyLight™ 755 (Ex-Max 754 nm/Em-Max 776 nm)

DyLight™ 755 has an excitation peak of 754 nm and an emission peak of 776 nm and is spectrally similar to Alexa Fluor™ 750. DyLight™ 755 is most commonly used in fluorescence micrscopy.

Excitation & emission spectra

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Key facts

Excitation maximum
754 nm
Emission maximum
776 nm
Optimal laser line
730-750
Optimal filter
780/60
Extinction coefficient
220000
Commercial antibodies
22.7k from 8 suppliers

DyLight 755 has an excitation maximum at 754 nm and an emission maximum at 776 nm, a Stokes shift of 22 nm. Its extinction coefficient is 220000 M⁻¹cm⁻¹. It is optimally excited by the 730-750 laser line.

Frequently asked questions

What is DyLight 755?
DyLight™ 755 has an excitation peak of 754 nm and an emission peak of 776 nm and is spectrally similar to Alexa Fluor™ 750. DyLight™ 755 is most commonly used in fluorescence micrscopy.
What laser excites DyLight 755?
DyLight 755 is optimally excited by the 730-750 laser line. Its excitation maximum is 754 nm.
What filter should I use to detect DyLight 755?
A 780/60 filter captures the peak of the DyLight 755 emission near 776 nm with minimal spillover.
How many antibodies are available conjugated to DyLight 755?
FluoroFinder tracks 22744 commercially available DyLight 755 conjugates, searchable by target, clone, host and application.