What laser excites Live/Dead Fix Near IR (780)?

Live/Dead Fix Near IR (780) is optimally excited by the 628-640 laser line. Its excitation maximum is 750 nm.

What filter should I use to detect Live/Dead Fix Near IR (780)?

A 780/60 filter captures the peak of the Live/Dead Fix Near IR (780) emission near 785 nm with minimal spillover.

How many antibodies are available conjugated to Live/Dead Fix Near IR (780)?

FluoroFinder tracks 3 commercially available Live/Dead Fix Near IR (780) conjugates, searchable by target, clone, host and application.

Live/Dead Fix Near IR (780)

Live/Dead Fix Near IR (780) (Ex-Max 750 nm/Em-Max 782 nm)

The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Near IR (780) has an excitation peak at 750 nm and an emission peak at 785 nm. In flow cytometry this probe is typically excited using the 633 nm laser.

Excitation & emission spectra

Key facts

Excitation maximum: 750 nm
Emission maximum: 785 nm
Optimal laser line: 628-640
Optimal filter: 780/60
Commercial antibodies: 3 from 1 supplier

Live/Dead Fix Near IR (780) has an excitation maximum at 750 nm and an emission maximum at 785 nm, a Stokes shift of 35 nm. It is optimally excited by the 628-640 laser line.

Frequently asked questions

What is Live/Dead Fix Near IR (780)?: The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Near IR (780) has an excitation peak at 750 nm and an emission peak at 785 nm. In flow cytometry this probe is typically excited using the 633 nm laser.
What laser excites Live/Dead Fix Near IR (780)?: Live/Dead Fix Near IR (780) is optimally excited by the 628-640 laser line. Its excitation maximum is 750 nm.
What filter should I use to detect Live/Dead Fix Near IR (780)?: A 780/60 filter captures the peak of the Live/Dead Fix Near IR (780) emission near 785 nm with minimal spillover.
How many antibodies are available conjugated to Live/Dead Fix Near IR (780)?: FluoroFinder tracks 3 commercially available Live/Dead Fix Near IR (780) conjugates, searchable by target, clone, host and application.