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StarBright Violet 475
StarBright Violet 475 (Ex-Max 405 nm/Em-Max 476 nm)
StarBright Violet 475 (SBV475) is a fluorescent polymer nanoparticle that has an excitation peak at 383 nm and an emission peak at 476 nm, spectrally comparable to BV480. The StarBright family fluorophores from Bio-Rad offers an alternative to the Horizon Brilliant™ polymer series from BD Biosciences including Brilliant™ UV and Brilliant™ Violet.
Excitation & emission spectra
Key facts
- Excitation maximum
- 405 nm
- Emission maximum
- 476 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Relative brightness
- 2 of 5
- Commercial antibodies
- 65 from 1 supplier
StarBright Violet 475 has an excitation maximum at 405 nm and an emission maximum at 476 nm, a Stokes shift of 71 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is StarBright Violet 475?
- StarBright Violet 475 (SBV475) is a fluorescent polymer nanoparticle that has an excitation peak at 383 nm and an emission peak at 476 nm, spectrally comparable to BV480. The StarBright family fluorophores from Bio-Rad offers an alternative to the Horizon Brilliant™ polymer series from BD Biosciences including Brilliant™ UV and Brilliant™ Violet.
- What laser excites StarBright Violet 475?
- StarBright Violet 475 is optimally excited by the 405 laser line. Its excitation maximum is 405 nm.
- What filter should I use to detect StarBright Violet 475?
- A 450/50 filter captures the peak of the StarBright Violet 475 emission near 476 nm with minimal spillover.
- How bright is StarBright Violet 475?
- StarBright Violet 475 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to StarBright Violet 475?
- FluoroFinder tracks 65 commercially available StarBright Violet 475 conjugates, searchable by target, clone, host and application.