About Indo-Lo (Ca2+free)

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Indo-1, AM is a ratiometric calcium indicator optimally excited by a 355nm UV laser or excitation source. After Indo-1, AM is loaded into live cells, it is hydrolyzed and available to bind cytoplasmic Ca2+. Probe bound to Ca2+ emit at ~400nm, while probe unbound to Ca2+ emits at ~475nm. Because of these unique spectral characteristics, it's the preferred calcium indicator for flow cytometry and is often frequently used in fluorescence live-cell microscopy.

Dyes similar to Indo-Lo (Ca2+free) based on emission

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AmCyan ATTO 390 ATTO 425 BOBO-1 Brilliant Violet 480 Cerulean CPN475 Blue eCFP LysoBrite Blue mBBR mCerulean3 Nucleolar-ID Green Spectrum Aqua StarBright Violet 475