405

Laser Line

450/50

Common Filter

436

Excitation Max

478

Emission Max

836

Antibodies


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About Brilliant Violet 480


Brilliant Violet™ 480 (BV480) is a green-emitting non-tandem polymer fluorophore that can be excited by the 405 nm violet laser and collected using a 525/40 bandpass filter. BV480 has an excitation peak at 436 nm and an emission peak at 478 nm, and provides an optimized alternative to BV510. Other dyes that are similar include Pacific Blue (ThermoFisher Scientific), and AmCyan (BD Biosciences). While BV480 can be detected on the same filter as BV510, its emmision profile reduces spillover into the BV605, BV650, and BV711 channels. Additionally BV480's excitation profile will reduce cross-laser excitation with the UV laser, resulting in less spillover into UV channels than BV510. These advantages make BV480 an optimal choice for flow cytometry panels using multiple reagents on violet and/or UV lasers. This dye is part of the Brilliant Violet dye line. BV510 and BV480 are the only dyes in the Brilliant Violet dye family that are not tandem fluorophores based off the BV421 polymer core, but they have nearly the same maximum excitation.


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