PromoFluor 405
PromoFluor 405 (Ex-Max 401 nm/Em-Max 422 nm)
PromoFluor 405 is a fluorophore with an excitation peak at 400 nm and an emission peak at 420 nm. It is equivalent to Alexa Fluor™ 405 and Pacific Blue in size, brightness and application. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 401 nm
- Emission maximum
- 422 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Relative brightness
- 2 of 5
- Quantum yield
- 0.968
PromoFluor 405 has an excitation maximum at 401 nm and an emission maximum at 422 nm, a Stokes shift of 21 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is PromoFluor 405?
- PromoFluor 405 is a fluorophore with an excitation peak at 400 nm and an emission peak at 420 nm. It is equivalent to Alexa Fluor™ 405 and Pacific Blue in size, brightness and application. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.
- What laser excites PromoFluor 405?
- PromoFluor 405 is optimally excited by the 405 laser line. Its excitation maximum is 401 nm.
- What filter should I use to detect PromoFluor 405?
- A 450/50 filter captures the peak of the PromoFluor 405 emission near 422 nm with minimal spillover.
- How bright is PromoFluor 405?
- PromoFluor 405 has a relative brightness of 2 out of 5 (quantum yield 0.968).