405

Laser Line

450/50

Common Filter

401

Excitation Max

421

Emission Max

22,054

Antibodies

2

Services


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About Alexa Fluor 405


Alexa Fluor® 405 (AF405, Alexa 405) is a blue-emitting synthetic fluorophore that can be excited by the 405 nm violet laser and captured with a 450/50 nm bandpass filter. AF405 has an excitation peak at 401 nm and an emission peak at 421 nm, and is spectrally similar to V450 (BD Biosciences), VioBlue® (Miltenyi Biotec), Cascade Blue™ (ThermoFisher Scientific), Pacific Blue™ (ThermoFisher Scientific), SuperBright 436 (ThermoFisher Scientific) and Brilliant Violet 421 (BioLegend/BD Biosciences). AF405 is most commonly used in flow cytometery, and fluorescence imaging applications. Additionally, this dye is known to be bright, photostable, and pH insensitive suits it well for immunohistochemistry applications and more specifically super-resolution microscopy using STORM. The Alexa Fluor dye family was originally developed by Molecular Probes, now a part of ThermoFisher Scientific. AlexaFluor is a registered trademark of Invitrogen, ThermoFisher Scientific. Alexa Fluor dyes come in a wide variety of modified formats that allow for easy conjugations or right out of the box use.


Popular Alexa Fluor 405 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
Novus Biologicals
NBP2-24867AF405
FC CD3
Human,Mouse
Mouse
IgG2a κ
OKT3
0.1 ml
$379
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Novus Biologicals
NBP1-30426AF405
ICC, IF,WB,FC,IHC-Fr,IHC CD3 epsilon
Mouse,Human
Mouse
IgG1
APA1/1
0.1 ml
$409
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Thermo Fisher Scientific
CD0326
FC CD3
Human
Mouse
IgG1
UCHT1
0.5 mL
$347
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Novus Biologicals
NBP2-34527AF405
ICC, IF,WB,ELISA,FC,IHC-Fr,IHC-P,IHC CD45
Human
Mouse
IgG1 κ
2B11
0.1 ml
$449
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Thermo Fisher Scientific
MHCD0826
FC CD8
Human
Mouse
IgG2a
3B5
500 μl
$340
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R&D Systems
FAB48881V-100UG
FC CD301
Human
Mouse
IgG2b
744812
100 μg
$335
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R&D Systems
FAB9929V-100UG
FC CD3
Human
Human
IgG1
Hu113
100 μg
$355
View
Novus Biologicals
NBP2-59677AF405
WB,FC,ICC, IF Mre11
Human,Mouse
Armenian Hamster
IgG
15B8.1E7.6
0.1 ml
$429
View
R&D Systems
FAB36551U-100UG
FC Cannabinoid Receptor II
Human
Mouse
IgG2b
352110
100 μg
$345
View
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