405

Laser Line

450/50

Common Filter

405

Excitation Max

421

Emission Max

2,748

Antibodies

2

Services


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About Brilliant Violet 421


Brilliant Violet™ 421 (BV421) is a violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor 405 and Cascade Blue. Other dyes that are considered similar include V450 (BD Biosciences), VioBlue® (Miletnyi Biotec), Cascade® Blue (ThermoFisher Scientific), Pacific Blue (ThermoFisher Scientific), SuperBright 436 (ThermoFisher Scientific), and Alexa Fluor® 405 (ThermoFisher Scientific). BV421 is very bright and is most commonly used for flow cytometry. Its photostablity, fixation stablity, and non-toxicity properties also make BV421 useful for cell sorting and live cell imaging applications. This dye is part of the Brilliant Violet dye line, and this dye specifically is one of the most popular in the line. The Brilliant Violet dye line itself is very popular because other dyes in the line also use the Brilliant Violet 421 core as the donor molecule, while varied acceptor molecules emit light at various points across the visible light spectrum. This allows for multiple dyes to be compatible while excited by the single laser. In cytometry, a user may populate all the available channels off their 405-violet laser with Brilliant Violet dyes.


Popular Brilliant Violet 421 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BioLegend
123132
FC,IHC-Fr F4/80
Mouse
Rat
IgG2a κ
BM8
500 μl
$396
View
BioLegend
329920
FC PD-1
Human,African Green Monkey,Baboon,Chimpanzee,Common Marmoset,Cynomolgus,Rhesus,Squirrel Monkey
Mouse
IgG1 κ
EH12.2H7
100 Tests
$402
View
BioLegend
353207
FC CCR7
Human,African Green Monkey,Baboon,Cynomolgus,Rhesus
Mouse
IgG2a κ
G043H7
25 Tests
$215
View
BioLegend
353208
FC CCR7
Human,African Green Monkey,Baboon,Cynomolgus,Rhesus
Mouse
IgG2a κ
G043H7
100 Tests
$407
View
BD Biosciences
562561
FC CD11c
Human
Mouse
IgG1 κ
B-ly6
100 Tests
$370
View
BioLegend
141717
FC,ICS,IHC-Fr Mannose Receptor
Mouse
Rat
IgG2a κ
C068C2
125 μl
$204
View
BioLegend
103134
FC CD45
Mouse
Rat
IgG2b κ
30-F11
50 μg
$259
View
BioLegend
317344
FC CD3
Human
Mouse
IgG2a κ
OKT3
100 Tests
$380
View
BioLegend
126419
ICS FoxP3
Mouse
Rat
IgG2b κ
MF-14
50 μg
$253
View
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