Brilliant Violet 421 Dye Profile

BD Biosciences™

405

Laser Line

450/50

Common Filter

405

Excitation Max

421

Emission Max

2500000

Extinction Coefficient

0.65

Quantun Efficiency

70000

Molecular Weight

About Brilliant Violet 421


Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad.



Spectra


Alternative Dyes to Brilliant Violet 421

Check to add to spectra viewer
StarBright Violet 440 SuperBright 436 SuperNova v428


Popular Brilliant Violet 421 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BioLegend
126419
ICS FOXP3
Mouse
Rat
IgG2b κ
MF-14
50 μg
$253
View
BioLegend
353207
FC CCR7
Human
Mouse
IgG2a κ
G043H7
25T
$215
View
BioLegend
329920
FC PD-1
Human
Mouse
IgG1 κ
EH12.2H7
100T
$402
View
BioLegend
353208
FC CCR7
Human
Mouse
IgG2a κ
G043H7
100T
$407
View
BioLegend
317344
FC CD3
Human
Mouse
IgG2a κ
OKT3
100T
$360
View
BioLegend
100228
FC,ICC CD3
Mouse
Rat
IgG2b κ
17A2
50 μg
$242
View
BioLegend
320124
ICS FOXP3
Human
Mouse
IgG1 κ
206D
100T
$435
View
BD Biosciences
562561
FC CD11c
Human
Mouse
IgG1 κ
B-ly6
100 Tests
$414
View
BioLegend
103134
FC,Spatial Biology CD45
Mouse
Rat
IgG2b κ
30-F11
50 μg
$242
View
BioLegend
141717
FC,Spatial Biology,ICS,IHC-Fr Mannose Receptor
Mouse
Rat
IgG2a κ
C068C2
125UL
$204
View
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Popular Brilliant Violet 421 Secondary Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BioLegend
405725
FC IgD
Mouse
Rat
IgG2a κ
11-26c.2a
50 μg
$280
View
BioLegend
348226
FC IgD
Human
Mouse
IgG2a κ
IA6-2
100T
$402
View
BioLegend
314516
FC IgM
Human
Mouse
IgG1 κ
MHM-88
100T
$402
View
BioLegend
410703
FC IgG Fc
Human
Rat
IgG2a κ
M1310G05
25T
$204
View
BioLegend
314515
FC IgM
Human
Mouse
IgG1 κ
MHM-88
25T
$198
View
BD Biosciences
562618
FC IgM
Human
Mouse
IgG1 κ
G20-127
50 Tests
$296
View
BioLegend
406410
FC,ICC IgG
Rabbit
Donkey
Ig
Poly4064
125UL
$204
View
BioLegend
348225
FC IgD
Human
Mouse
IgG2a κ
IA6-2
25T
$190
View
BioLegend
406518
FC IgM
Mouse
Rat
IgG2a κ
RMM-1
500UL
$380
View
BD Biosciences
562581
FC IgG
Human
Mouse
IgG1 κ
G18-145
50 Tests
$296
View
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Brilliant Violet 421 Conjugation Services


Product Type Company/SKU Applications Details
Conjugation Service BD Biosciences
BD-CONJ-BV421
N/A View
Conjugation Service BioLegend
BL-CONJ-BV421
N/A View