DyLight 405
DyLight™ 405 (Ex-Max 400 nm/Em-Max 420 nm)
DyLight™ 405 has an excitation peak at 400 nm and an emission peak at 420 nm and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. DyLight™ 405 is most commonly used in flow cytometery and fluorescence microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 400 nm
- Emission maximum
- 420 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Relative brightness
- 2 of 5
- Extinction coefficient
- 30000
- Commercial antibodies
- 19.7k from 15 suppliers
DyLight 405 has an excitation maximum at 400 nm and an emission maximum at 420 nm, a Stokes shift of 20 nm. Its extinction coefficient is 30000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is DyLight 405?
- DyLight™ 405 has an excitation peak at 400 nm and an emission peak at 420 nm and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. DyLight™ 405 is most commonly used in flow cytometery and fluorescence microscopy applications.
- What laser excites DyLight 405?
- DyLight 405 is optimally excited by the 405 laser line. Its excitation maximum is 400 nm.
- What filter should I use to detect DyLight 405?
- A 450/50 filter captures the peak of the DyLight 405 emission near 420 nm with minimal spillover.
- How bright is DyLight 405?
- DyLight 405 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to DyLight 405?
- FluoroFinder tracks 19701 commercially available DyLight 405 conjugates, searchable by target, clone, host and application.